Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis?

AIM: Colon cancer is one of the main health problems worldwide. Cancer stem cells (CSCs) are referred to as tumor-initiating involved in tumor heterogeneity and dormancy. CSCs can cause drug resistance, metastasis, recurrence primary metastatic cancers. The interactions survival trends colon with other may be an alternative route for effective treatment. In our study, we aimed evaluate effects asteoside on cell properties, apoptotic inflammatory processes (HCT-116) (Colo-741) CSCs. METHERIALS AND METHODS: were obtained from both types lines MINIMACS system using anti-CD133 reagent. Metastatic Colo-741 non-metastatic HCT-116 CD133+ CD133- cultured or without Acteoside 48 hours. Expressions Caspase-3, Bcl, Bax, Fas-L apoptosis, IL-1β, TNF-α, IL-6, IL8 IL-10 inflammation analyzed by indirect immunocytochemistry technique performing H-Score. Changes morphology examined under inverted microscope. RESULTS CONCLUSION: It was observed that Oct-4 expression decreased after acteocyte administration cells. addition, increased intensity Bax/Bcl ratio caspase 3 expression. While acteoside did not immunohistochemically affect COLO-741 cells, it contributed process changing ratio. When evaluated, number